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| 1. |  | CORREIA, C. N.; SILVA, R. L. de O.; FERREIRA NETO, J. R. C.; ALMEIDA, J. C. Q. P. de; SILVA, M. D. da; ROCHA, M. de M.; FREIRE FILHO, F. R.; BENKO-ISEPPON, A. M.; KIDO, E. A. Desenvolvimento de marcadores moleculares AFLP para mapeamento genético em feijão-caupi. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 5., 2009, Guarapari. O melhoramento e os novos cenários da agricultura: anais. Vitória: Incaper, 2009. 4 p. 1 CD-ROM. (Incaper. Documentos, 11).| Biblioteca(s): Embrapa Meio-Norte. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
13/11/2019 |
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Data da última atualização: |
13/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
TOMASI, J. de C.; DEGENHARDT-GOLDBACH, J.; GRUNENNVALDT, R. L.; SANTOS, G. D. dos; VICENTE, V. A.; FRANCISCON, L.; BONA, C.; QUOIRIN, M. |
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Afiliação: |
Jéssica de Cássia Tomasi, UFPR; JULIANA DEGENHARDT GOLDBACH, CNPF; Renata Lucia Grunennvaldt, UFPR; Germana Dávila dos Santos, UFPR; Vania Aparecida Vicente, UFPR; LUZIANE FRANCISCON, CNPF; Cleusa Bona, UFPR; Marguerite Quoirin, UFPR. |
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Título: |
In vitro establishment of shoot meristems of Ilex paraguariensis and identification of endophytic bacteria. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of Forestry Research, v. 30, n. 5, p. 1765-1777, Oct. 2019. |
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DOI: |
10.1007/s11676-018-0763-x |
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Idioma: |
Inglês |
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Conteúdo: |
Yerba mate (Ilex paraguariensis A. St.-Hil.) is a species of great economic, social and environmental importance for the southern regions of Brazil, Uruguay and Argentina. Currently the most diverse products are obtained from mate leaves, including mate tea. The objective of this study was to establish shoot meristem cultures (meristematic dome and a few primordia) of elite clones and identify the endophytic bacteria present in the explants. We tested the effect of clones (F1, F2, A03 and A07), culture media (MS, 1/2MS, 1/4MS and WPM), cytokinins (kinetin, BA and 2iP), activated charcoal (1, 2 and 3 g L-1), and disinfecting agent (sodium hypochlorite and mercuric chloride) on in vitro establishment. F1 and F2 clones were the most responsive for shoot meristem in vitro culture. WPM medium supplemented with 8.8 µM 2iP, 0.2 µM NAA and 3 g L-1 activated charcoal was the most suitable for the in vitro establishment of the F1 clone. No phytotoxic effect of the disinfectant was observed and some meristems sprouted. The isolated endophytic bacterium was identified for the first time in yerba mate as Agrobacterium larrymoorei. To conclude, we were able to establish in vitro culture of yerba mate using meristems as explants but the tissues were not free of endophytic microorganisms which could interfere with explant response. |
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Palavras-Chave: |
2iP; Activated charcoal; Endophytic bacteria; Erva mate. |
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Thesagro: |
Cultura de Tecido; Ilex Paraguariensis; Oxidação. |
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Thesaurus NAL: |
Agrobacterium larrymoorei; Oxidation; Tissue culture; Yerba mate. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 02375naa a2200349 a 4500
001 2114253
005 2019-11-13
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1007/s11676-018-0763-x$2DOI
100 1 $aTOMASI, J. de C.
245 $aIn vitro establishment of shoot meristems of Ilex paraguariensis and identification of endophytic bacteria.$h[electronic resource]
260 $c2019
520 $aYerba mate (Ilex paraguariensis A. St.-Hil.) is a species of great economic, social and environmental importance for the southern regions of Brazil, Uruguay and Argentina. Currently the most diverse products are obtained from mate leaves, including mate tea. The objective of this study was to establish shoot meristem cultures (meristematic dome and a few primordia) of elite clones and identify the endophytic bacteria present in the explants. We tested the effect of clones (F1, F2, A03 and A07), culture media (MS, 1/2MS, 1/4MS and WPM), cytokinins (kinetin, BA and 2iP), activated charcoal (1, 2 and 3 g L-1), and disinfecting agent (sodium hypochlorite and mercuric chloride) on in vitro establishment. F1 and F2 clones were the most responsive for shoot meristem in vitro culture. WPM medium supplemented with 8.8 µM 2iP, 0.2 µM NAA and 3 g L-1 activated charcoal was the most suitable for the in vitro establishment of the F1 clone. No phytotoxic effect of the disinfectant was observed and some meristems sprouted. The isolated endophytic bacterium was identified for the first time in yerba mate as Agrobacterium larrymoorei. To conclude, we were able to establish in vitro culture of yerba mate using meristems as explants but the tissues were not free of endophytic microorganisms which could interfere with explant response.
650 $aAgrobacterium larrymoorei
650 $aOxidation
650 $aTissue culture
650 $aYerba mate
650 $aCultura de Tecido
650 $aIlex Paraguariensis
650 $aOxidação
653 $a2iP
653 $aActivated charcoal
653 $aEndophytic bacteria
653 $aErva mate
700 1 $aDEGENHARDT-GOLDBACH, J.
700 1 $aGRUNENNVALDT, R. L.
700 1 $aSANTOS, G. D. dos
700 1 $aVICENTE, V. A.
700 1 $aFRANCISCON, L.
700 1 $aBONA, C.
700 1 $aQUOIRIN, M.
773 $tJournal of Forestry Research$gv. 30, n. 5, p. 1765-1777, Oct. 2019.
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